Figure 3. Effects of agonist treatment and hypotonic solution on neurotoxicity.

Neurotoxicity was assessed using % PI-uptake 24 h after treatment. Neuronal cultures were exposed as per bar diagram labels for 20 min for NMDA and veratridine and for 10 min for low Na BPS. MK-801 prevented PI-uptake related to NMDA channel activation, both during NMDA-treatment and during low Na⁺ induced neuronal swelling. In the latter case, neurotoxicity was presumably a secondary consequence of swelling-induced glutamate release (and hence, of hyper-activation of NMDA channels). Error bars are standard deviations based on n = 6-9 for each treatment.