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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Nat Genet. 2013 Jul 21;45(9):1083–1087. doi: 10.1038/ng.2705

Figure 2. Comparison of mosaic and SHIMS sequence assemblies across one region of human X chromosome.

Figure 2

a, Triangular dot-plot highlights sequence similarities within mosaic (multi-haplotype) assembly. Each dot represents 100% identity within a window of 100 nucleotides; direct repeats appear as horizontal lines, inverted repeats as vertical lines, and palindromes as vertical lines that nearly intersect the baseline; gaps are indicated by gray shading. Black arrows immediately below plots denote positions and orientations of amplicons. Further below, sequenced BACs from CTD, RP-11, and RP-13 libraries (each from a different individual) contributing to the assembly are depicted as orange, green, and purple bars, respectively; each bar reflects extent and position within assembly of finished sequence for that BAC. (As per the human genome assembly standard, finished-sequence overlaps between adjoining BACs are limited to 2 kb.) GenBank accession numbers in Supplementary Table 1. b, SHIMS assembly of same region. All BACs derive from RP-11 library (one male) and are fully sequenced; each BAC’s finished sequence extensively overlaps those of adjoining BACs.