Correlated fluorescence and x-ray images of Schizosaccharomyces pombe. (A) Cryo-immobilized, high-aperture wide-field fluorescence image of S. pombe. The vacuoles were stained with CMFDA (5-chloromethyl fluorescein diacetate). (B) Soft X-ray tomography of the same cell shown in an orthoslice from the tomgraphic reconstruction. (C) Segmented vacuoles were overlaid on the orthoslice. (D) The completely segmented cell overlaid on the orthoslice. The segmented cell. Key: nucleus, blue; nucleolus, orange; mitochondria, grey; vacuoles, white; lipid-rich vesicles, green. Scale bar = 2 μm.