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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Nat Neurosci. 2013 Jul 28;16(9):1275–1283. doi: 10.1038/nn.3478

Figure 6. Cre-mediated CB1 deletion effects on state dependent iLTD.

Figure 6

Breeding RGS9-cre mice with floxed CB1 mice produced selective deletion of CB1 from MSNs and, subsequent to AAV2-DIO-ChR2-mCherry injection, also expressed mCherry and ChR2 in MSNs (a, b). a, LFS delivery via the stimulating electrode while holding MSNs at −60 mV failed to depress MSN-MSN oIPSC (blue circles) and eIPSC (black circles) amplitude. b, LFS delivery while holding MSNs at −80 mV did not depress MSN-MSN oIPSC amplitude (blue circles), but did depress eIPSC amplitude (black circles). Breeding PV-cre mice with CB1 floxed mice produced selective deletion of CB1 from FSIs and, subsequent to AAV2-DIO-ChR2-mCherry injection, also expressed mCherry and ChR2 in FSIs (c, d). c, LFS delivery while holding MSNs at −60 mV failed to depress FSI-MSN oIPSC amplitude (blue circles), but did depress eIPSC amplitude (black circles). d, LFS delivery while holding MSNs at −80 mV failed to depress FSI-MSN oIPSC amplitude (blue circles), but did depress eIPSC amplitude (black circles). Scale bars: 300 pA (vertical), 100 ms (horizontal). Inset current traces show IPSCs before (dark) and 20 min after (light) the iLTD induction protocol. All error bars represent SEM.