Figure 2.

Plot of the differences between the 13C chemical shifts of assigned carbon positions in CCl4 and in various lipid phases

Comparison of chemical shift differences (Δδ, defined as the chemical shift in CCl₄ minus the chemical shift in the conditions described) of assigned cholesterol carbons in DMPC-d₅₄ at 40 w/w% (black) and 20 w/w% (dark grey) hydration, and MO at 40 w/w% (white) and 20 w/w% (light grey) hydration. All DMPC-d₅₄ and MO samples contained 10 mol% cholesterol and data were recorded at 30°C. At 20 w/w% hydration the DMPC-d₅₄ and MO samples were both in a lamellar phase. At 40 w/w% hydration the DMPC-d₅₄ sample was in a lamellar phase whereas the MO sample was in a cubic phase. Chemical shifts were calibrated to C18 at 11.84ppm, and are accurate to ± 0.05ppm in DMPC-d₅₄ and ± 0.03ppm in MO.