Figure 3.

GD1a/GT1b-2b mAb inhibits neurite outgrowth of DRG neurons in Schwann cell-depleted culture. A, DRG cultures with and without AraC treatment are then double labeled with neuronal (β-III-tubulin; green) and Schwann cell (S100; red) markers showing that AraC-treated cultures have almost complete elimination of Schwann cells. Scale bar, 50 μm. B, GD1a/GT1b-2b mAb induces significant inhibition of neurite outgrowth compared with sham Ab-treated neurons in Schwann cell-depleted cultures (AraC treated). *p < 0.05. Control, Cultures without sham or anti-ganglioside Ab treatment.