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. 2011 Feb 2;31(5):1664–1675. doi: 10.1523/JNEUROSCI.3829-10.2011

Figure 7.

Figure 7.

GD1a/GT1b-2b mAbs induce activation of RhoA by engaging specific cell-surface gangliosides. A, B, GD1a/GT1b-2b mAb induce RhoA activation in DRG neurons, which is concentration (A) and time (B) dependent. RhoA activation was assessed at 30 min after antibody incubation in studies shown in A, and antibody concentration was 100 μg/ml for studies shown in B. OD, Optical density. *p < 0.05. C, Increased amount of GTP-bound RhoA was immunoprecipitated in GD1a/GT1b-2b-treated NG108–15 cells compared with sham Ab-treated cells. GST-RBD, Glutathione S-transferase/Rho-binding domain. D, GD1a/GT1b-2b mAb induces significant activation of RhoA in wild-type (black bars; WT) but not in Galgt1-null (gray bars) DRG neurons. Sham Ab did not induce RhoA activation in wild-type or Galgt1-null DRG neurons. *p < 0.05.