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. 2011 Feb 23;31(8):3032–3043. doi: 10.1523/JNEUROSCI.4345-10.2011

Figure 4.

Figure 4.

Determination of Dhh-Cre recombination efficiency in floxed N-cadherin and β-catenin mice. A, SCs were isolated and analyzed for N-cadherin expression from Dhh−/− Flox-N-cad+/− and Dhh+/− Flox-N-cad/Flox-N-cad mice at E15 (littermates). Although the expression of N-cadherin was still detectable, it was significantly diminished. B, Expression of N-cadherin in SCs from E18 littermates was essentially undetectable (similar to background fluorescence). C, SCs were isolated and analyzed for β-catenin expression from Dhh−/− Flox-β-cat+/− and Dhh+/− Flox-β-cat/Flox-β-cat mice at E15 (littermates). Similar to N-cadherin, the expression of β-catenin was still detectable but was significantly diminished. D, Expression of β-catenin in SCs from E18 littermates was essentially undetectable (similar to background fluorescence). N-cadherin and β-catenin staining is identified in green and cell nuclei are in blue (DAPI). Scale bars, 20 μm.