Figure 1.

Cochlin is produced and secreted by FDCs in the spleen and the lymph nodes.

A. Spleen and lymph node lysates from WT and Coch^−/− mice were analyzed by WB.

B.C. Lymph node and spleen sections from WT mice were co-stained for cochlin and B cell (B220) (objective lens 10×) (B) or FDCs (CR2.1) (objective lens 20 ×) (C).

D.E. Primary FDCs were isolated from WT and Coch^−/− mice. Lysates from in vitro cultured FDCs were analyzed by WB (D). Culture media were subjected to anti-cochlin IP and analyzed by WB (E). Spleen lysates from WT and Coch^−/− mice, as well as lysates from 293T cells expressing cochlin were used as controls.

See also Figure S1.