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. 2011 Jul 6;31(27):9933–9944. doi: 10.1523/JNEUROSCI.1062-11.2011

Figure 2.

Figure 2.

Neuromorphological characterization of the DG in 8-week-old Ift20 mutant (right panels) and wild-type (left panels) mice. A, B, NeuN (red) immunofluorescence shows preserved layering in hippocampal subregions (CA1, CA2, CA3, and DG) in Ift20 mutant and wild-type mice. C, D, Hoechst (white) nuclear staining in the GCL of the DG reveals no differences between Ift20 mutant and wild-type mice. E, F, NeuN (white) staining shows similar neuronal density in the GCL in both genotypes. G, H, Ift20 mutants show similar patterns of GFAP+ (green) astrocytes in the DG, hilus, and molecular layer (Mol) of the DG compared with wild-type mice. I, J, GFAP (green) and NESTIN (red) coexpression in cells from the SGZ of the DG indicates conserved morphology and distribution along the GCL. Section thickness: A–J, 30 μm. Scale bars: A, B, 500 μm; C–F, 50 μm; G, H, 200 μm; I, J, 20 μm.