Figure 4.

Proliferation in the SVZ of the LVs is similar in Ift20 mutant (right panels) and wild-type mice (left panels). A, B, BrdU staining in 8-week-old mice after a 2 h BrdU pulse (100 mg kg⁻¹ body weight) showed no evident genotypic differences in proliferation in the SVZ (CPu, caudate–putamen) (quantified in C). D, E, Staining with the proliferative marker PCNA (red) confirmed that proliferation in the SVZ is similar in Ift20 mutants and wild-type mice (quantified in F). G, H, A 5 d BrdU injection protocol (twice daily, 12 h apart, at 50 mg kg⁻¹ body weight) was used in 8-week-old Ift20 mutant and wild-type mice. Double labeling of BrdU (green) and NeuN (red) in the olfactory bulb (OB) 4 weeks after BrdU injection in Ift20 mutant and wild-type mice shows similar distribution of adult newborn neurons in the GCL in the two groups. I, J, BrdU (green) and NeuN (red) double staining in the GCL of the OB 4 weeks after BrdU injection showed no genotypic differences in neurogenesis (quantified in K). Section thickness: A, B, D, E, G–J, 30 μm. Scale bars: A, B, 500 μm; D, E, G, H, 200 μm; I, J, 50 μm. Data are expressed as mean values ± SEM.