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. Author manuscript; available in PMC: 2013 Aug 31.
Published in final edited form as: Methods Mol Biol. 2012;914:47–63. doi: 10.1007/978-1-62703-023-6_4

Fig. 6.

Fig. 6

(a) 1H 1D spectrum of neotame; (b) STD signal resulting from binding of neotame to the VFTM of T1R2. Only resonances at lower frequencies from the water signal are shown; these signals arise from binding of neotame to the purified amino terminal domain of the hT1R2 subunit. Data were processed by NMRPipe software, and subtraction was performed by Newton (in-house) software in the frequency domain.