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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Kidney Int. 2013 May 1;84(3):468–481. doi: 10.1038/ki.2013.149

Figure 2. Functional EpoR in rat kidney. EpoR activity was assessed by EPO-induced activation of downstream effectors in rat kidney assayed by determination of phosphorylation of Erk and Jak2.

Figure 2

Figure 2

Figure 2

Renal artery was surgically isolated and EPO in oxygenated 1640 culture media (40 ml of 100 IU/ml) was continuously perfused for 15 minutes with Intravenous infusion system (Instech Laboratories, Inc, Plymouth Meeting, PA). After 15 minutes, kidneys were harvested for further study. (A) Left panel shows representative blots for phospho-Erk (P-Erk) and total Erk (T-Erk), and phospho-Jak2 (P-Jak2) and total Jak2 (T-Jak2). Summarized data (means ± SD) are shown in the right panel. (B) Representative immunohistochemistry for P-Erk and T-Erk in the kidney. (C) Representative immunohistochemistry for P-Jak2 and T-Jak2 in the kidney.