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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Kidney Int. 2013 May 1;84(3):468–481. doi: 10.1038/ki.2013.149

Figure 3. Genetic levels of Klotho correlates with levels of EpoR protein and mRNA expression in the kidney.

Figure 3

(A) sixty mg protein of total kidney Lysate was loaded onto each lane, subjected to SDS-PAGL, and immunoblotted for EpoR and Klotho. EpoR and Klotho protein expression in mouse kidney (detected with KM206 and M-20 antibody respectively). Kl-/-= Klotho knock-out mice; WT=Wild type mice; Tg-Kl= transgenic Klotho overexpressing mice. Left panel: Typical blot. Right: Summarized data as means ± SD. (B) EpoR transcript level in mouse kidney by quantitative RT-PCR (qRT-PCR) expressed relative to wild type mouse and summarized as means ± SD. (C) Acute Klotho injection up-regulates EpoR protein and mRNA expression in rat kidney. Full length extracellular domain of recombinant mouse Klotho protein (rMKl) was injected intraperitoneally 0.01 mg/Kg body weight) once into rats and kidneys were harvested 24 hours later. EpoR protein was examined by immunoblot with M-20 and EpoR mRNA by qRT-PCR respectively. Typical immunoblot of EpoR protein (left panel), summarized data of EpoR protein (middle panel) and EpoR transcript by qRT-PCR (right panel). All bars and error bars are mean and SD. *p<0.05, **p<0.01 ANOVA followed by Student-Newman-Keuls test.