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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Kidney Int. 2013 May 1;84(3):468–481. doi: 10.1038/ki.2013.149

Figure 5. Klotho regulates EpoR protein, mRNA, and signaling in NRK cells.

Figure 5

NRK cells were treated with Klotho in cell culturere medium containing 10% FBS for 24 hours. (A) Typical immunoblot for EpoR (left panel) and data summarized as means ± SD (right panel). (B). EpoR mRNA by qPCR. Cyclophilin served as control. Means ± SD of 4 independent experiments. **p< 0.01 unpaired Student t-test. (C).NRK cells were incubated with Klotho protein in medium containing 10% FBS for 24 and 48 hours. Total lysates were immunoblotted for phosphorylated (P-Jak2) and total Jak2 (T-Jak2) (left panel). Summarized data as means ± SD (right panel) of 4 independent experiments. *p<0.05, **p<0.01 ANOVA followed by Student-Newman-Keuls test.