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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Kidney Int. 2013 May 1;84(3):468–481. doi: 10.1038/ki.2013.149

Figure 8. Effect of overexpression of EpoR on H2O2 toxicity in NRK cells.

Figure 8

(A) NRK cells were transfected with HA-tagged EpoR and growth in cell culture medium containing 10% FBS. Total cell lysates were subjected to SDS-PAGE followed by immunobloting with anti-HA and anti-EpoR. Typical blot (left panel) and summarized data as means ± SD (right panel). *p<0.05 **p<0.01 by Student t test. (B) HA-EpoR overexpression and EPO-induced phosphorylation of Jak2. Typical blot (left panel) and summarized data as means ± SD (right panel). **p<0.01 by Student t test. (C) Effect of HA-EpoR overexpression on phosphorylation and translocation of Stat5. P-Stat5=phosph-Stat5. (D) Effect of EpoR overexpression on H2O2 cytotoxicity in NRK cell's. Means ± SD from 4 independent experiments *p<0.01 by ANOVA followed by Student-Newman-Keuls test.