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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Biochem Biophys. 2013 Sep;67(1):67–73. doi: 10.1007/s12013-013-9634-4

Figure 2.

Figure 2

Effects of ubiquitin overexpression on the mouse nervous system. (A) Representative immunoblot of ubiquitin levels from the brains of 6-week-old wild type mice (wt) and wild type mice containing the Thy1-ubiquitin transgene (wt + TgUb). Gapdh was used as a loading control. (B) Quantitative PCR was performed on reversetranscribed RNA isolated from brains of 6-week-old wild type (wt) and wild type ubiquitin transgenic mice (wt+TgUb). Data are shown as mean ± SE, where n = 3 animals per genotype. (C) Tibialis anterior muscles were prepared from 6-week-old wild type mice (wt) and wild type mice containing the Thy1-ubiquitin transgene (wt + TgUb). Muscles were stained for neurofilaments (green) and acetylcholine receptors (red). Arrows indicate neurofilament accumulations.