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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Biochem Biophys. 2013 Sep;67(1):103–110. doi: 10.1007/s12013-013-9633-5

Fig. 1.

Fig. 1

UBE2W exists in a monomer–dimer equilibrium. a Size exclusion elution profiles for UBE2W-WT (blue) and UBE2D3 (red) detect both a monomer and dimer for UBE2W, but only a monomer of UBE2D3. b Mutation of V30 and D67 to lysine in UBE2W (UBE2W-KK) (red) shifts the population of E2 toward a single monomeric peak as compared to UBE2W-WT (blue) c A C-terminal truncation mutant (UBE2W-131D) (red) elutes as both a monomer and dimer, similar to UBE2W-WT (blue). d The ‘KK’ mutation in the UBE2W-131Δ (UBE2W-131Δ-KK) (red) truncation background shifts the E2 population to a single monomeric peak when compared with the UBE2W-131Δ (blue). All size exclusion injections were performed on a column with a relative void volume of −5 mL