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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cell Biochem Biophys. 2013 Sep;67(1):91–101. doi: 10.1007/s12013-013-9626-4

Figure 2.

Figure 2

Structural representation of an activity-based probe (ABP), which is comprised of a targeting sequence that directs the probe to the targeted enzyme, a reactive chemical warhead which binds irreversibly to the enzyme’s catalytic residues, and a tag for identification or visualization of probe-bound enzymes. Biotin-tagged ABPs can be used for affinity purification and mass spectrometry-based identification of probe targets, as well as for visualization of these targets by streptavidin affinity blotting. Radiolabeled and fluorescent ABPs can be used to visualize target proteins by in-gel fluorescence. Additionally, cell-permeable fluorescent ABPs can be used to monitor enzyme activity in living cells or tissue samples via fluorescence microscopy.