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. Author manuscript; available in PMC: 2014 Jun 24.
Published in final edited form as: Immunol Lett. 2013 Jun 24;153(0):15–21. doi: 10.1016/j.imlet.2013.06.003

Figure 3.

Figure 3

Rag−/− BALB/c hosts were transferred with a 1:1 mixture of wt and SAP−/− B cells loaded with cell trackers CMRA and CFSE, respectively. B cells numbers of recipient spleens were analyzed 90 minutes (A) or 60 hours (B) after cell transfers. Data is representative of two independent experiments. A portion of the donor B cell mixture was in vitro cultured in complete media at 37°C for 6 hours, then viability was tested by AnnexinV staining and DAPI uptake (C). Flow cytometric analysis of splenic B cell populations in wt and SAP−/− BALB/c mice (D); upper panel: gated on DAPI- lymphocytes, lower panel: gated on DAPI-B220+CD93- lymphocytes. Data is representative of multiple independent staining experiments of at least 2-3 animals per group in each time.