Table 3.

The primers and product size for PCR-SSP genotyping of HLA-DRB1 and HLA-DQB1.

	Primer sequence (5′ to 3′)		Size of PCR products (bp)
	For	Rev
DRB1 allele
01	TTGTGGCAGCTTAAGTTTGAAT	CTGCACTGTGAAGCTCTCAC	255
03	TACTTCCATAACCAGGAGGAGA	TGCAGTAGTTGTCCACCCG	151
04	GTTTCTTGGAGCAGGTTAAACA′	CTGCACTGTGAAGCTCTCAC	260
07	CCTGTGGCAGGGTAAGTATA	CCCGTAGTTGTGTCTGCACAC	232
08	AGTACTCTACGGGTGAGTGTT′	CTGCAGTAGGTGTCCACCAG	214
09	GTTTCTTGAAGCAGGATAAGTTT	CCCGTAGTTGTGTCTGCACAC	236
10	CGGTTGCTGGAAAGACGCC	CTGCACTGTGAAGCTCTCAC	204
11	GTTTCTTGGAGTACTCTACGTC	CTGGCTGTTCCAGTACTCCT	176
12	AGTACTCTACGGGTGAGTGTT	CACTGTGAAGCTCTCCACAG	248
13	ACTTCCATAACCAGGAGGAGA	CCCGCTCGTCTTCCAGGAT	130
14	GTTTCTTGGAGTACTCTACGTC	TCTGCAATAGGTGTCCACCT	224
15	TCCTGTGGCAGCCTAAGAG	CCGCGCCTGCTCCAGGAT	197
16	TCCTGTGGCAGCCTAAGAG	AGGTGTCCACCGCGGCG	223
DQB1 allele
0201	GTGCGTCTTGTGAGCAGAAG	GCAAGGTCGTGCGGAGCT	205
0301/4	GACGGAGCGCGTGCGTTA	AGTACTCGGCGTCAGGCG	122
0302/3	GACGGAGCGCGTGCGTCT′	AGTACTCGGCGTCAGGCG	122
0303	GACGGAGCGCGTGCGTCT	CTGTTCCAGTACTCGGCGT′	129
0304	GACGGAGCGCGTGCGTTA	CTGTTCCAGTACTCGGCGG	129
0401	CACCAACGGGACCGAGCT	GGTAGTTGTGTCTGCATACG	200
0402	CACCAACGGGACCGAGCG	GGTAGTTGTGTCTGCATACG	200
0501	CGGAGCGCGTGCGGG	GCTGTTCCAGTACTCGGCAA	128
0502	TGCGGGGTGTGACCAGAC	TGTTCCAGTACTCGGCGCT	117
0503	TGCGGGGTGTGACCAGAC	GCGGCGTCACCGCCCGA	87
0504	GTGCGGGGTCTGACCAGAT	TGTTCCAGTACTCGGCGCT	118
0601	GCCATGTGCTACTTCACCAAT	CACCGTGTCCAACTCCGCT	198
0602	CGTGCGTCTTGTGACCAGAT	GCTGTTCCAGTACTCGGCAT	121
0603/8	GGAGCGAGTGCGTCTTGTA	GCTGTTCCAGTACTCGGCAT	127
0604	CGTGTACCAGTTTAAGGGCA	GCAGGATCCCGCGGTACC	254

Note: For: former; Rev: reverse.