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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Int J Radiat Biol. 2009 Jun;85(6):523–531. doi: 10.1080/09553000902883810

Figure 2.

Figure 2

Sensitivity of fibroblasts to ionizing radiation at reduced oxygen concentrations. (A) Clonogenic survival of FANCD2-deficient (PD20) and wild-type complemented (PD20-wtD2) cells exposed to anoxia (0% oxygen) for 1 hour. (B) Clonogenic survival of FANCA-deficient PD220 fibroblasts and wild-type complemented cells exposed to anoxia (0% oxygen) for 1 hour. (C) Clonogenic survival of PD20 cells as in panel A, except that incubation under anoxia was extended for 1 hour after irradiation. (D) Clonogenic survival as in panel A, except that a gas mixture containing 3% oxygen instead of 0% oxygen was used. (E) Oxygen enhancement ratios (OER) calculated at the 10% survival level using the data shown in Figure 1B and 2A. Data points in panels A–D represent means +/− standard error based on at least three independent repeat experiments with all gassing and irradiation carried out at room temperature. Radiation survival curves and p-values were derived as described for Figure 1.