Deletion of BCR1 results in filamentous and invasive growth of opaque cells. A. Colony and cellular images of the WT (SN250α), bcr1/bcr1 mutant and bcr1/bcr1+BCR1p-BCR1 reconstituted strain. Wh, white phase; op, opaque phase. Scale bar, 10 μm. B. Invasive growth of the WT (SN250α), bcr1/bcr1 mutant and bcr1/bcr1+BCR1p-BCR1 reconstituted strain. White and opaque cells (~6 × 104) were spotted on Lee’s glucose medium plates and incubated at 25°C for 3 days. C. Expression of opaque (WOR1 and OP4) or filamentous (HWP1 and ECE1) enriched genes in the bcr1/bcr1 mutant analyzed by Northern blot.