ChIP-PCRs of the promoter regions of Bcr1-regulated genes. EFG1(b1), Bcr1 binding site 1 (−7188 to −7422); EFG1(b2), Bcr1 binding site 2 (−2220 to −1886). PCR products were detected after 27 cycles. The primers were designed according to the Bcr1 binding sites reported by Nobile et al., 2012. The ChIP-PCR of the HGC1 promoter, which was known not to be bound by Bcr1 (Nobile et al., 2012), served as the negative control. White and opaque cells of the strain CJN1787a (MTLa/α Δ::SAT1 BCR1/BCR1-Myc) were used for the experiments.