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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Mol Microbiol. 2013 Jul 12;89(4):732–750. doi: 10.1111/mmi.12310

Fig. 4.

Fig. 4

Quantitative mating assay indicates that filamentous opaque cells of the bcr1/bcr1 mutant are mating-competent. MTLa/a and MTLα/α cells in white or opaque phase were mixed and cultured on Lee’s glucose plates for 4 days at 25°C. About 1 × 106 cells of each mating mixture were resuspended and plated onto selectable SCD plates. A representative selectable plate (depleted with uridine and arginine) of each cross was imaged. The mating efficiencies were calculated and shown below the images. A colony image indicating the phase of experimental cells used for mating was also shown (top left corner). A. Mating between the WT opaque tester (GH1012, MTLa/a, ura3-) and white cells of the WT (MTLα/α, arg4-), bcr1/bcr1 mutant (MTLα/α, arg4-) and bcr1/bcr1+BCR1p-BCR1 reconstituted strain (MTLα/α, arg4-). B. Mating between the WT opaque tester (GH1012, MTLa/a, ura3-) and opaque cells of the WT (MTLα/α, arg4-), bcr1/bcr1 mutant (MTLα/α, arg4-) and bcr1/bcr1+BCR1p-BCR1 reconstituted strain (MTLα/α, arg4-).