Regulation of white and opaque cell filamentation by Bcr1, Cup9, Nrg1, Czf1 and Rfg1. White and opaque cells were plated onto Lee’s glucose medium with different pH (4.0 to 8.0). The colony and cellular images of the WT (SN250α), bcr1/bcr1, cup9/cup9, nrg1/nrg1 and rfg1/rgf1 were taken after incubation at 25°C for 5 days. The czf1/czf1 mutant was incubated at 25°C for 8 days and imaged. Wh, white colonies; Op, opaque colonies. Cellular morphology of the mutants is shown in Fig. S3.