TABLE I.
Variations in Ct values of Real Time PCR in different runs with rotavirus plasmid standards
| Plasmid Dilutions | Cq values
|
Mean +/− SDa (CVb) | |||||
|---|---|---|---|---|---|---|---|
| Run 1 | Run 2 | Run 3 | Run 4 | Run 5 | Run 6 | ||
| 1 | 6.42 | 6.61 | 6.51 | 6.34 | 6.4 | 6.27 | 6.43 +/− 0.1211 (1.89) |
| 2 | 9.56 | 9.69 | 9.17 | 9.4 | 8.93 | 9.23 | 9.33 +/− 0.2768 (2.97) |
| 3 | 12.53 | 12.94 | 13.02 | 12.72 | 12.38 | 12.59 | 12.70 +/− 0.2465 (1.94) |
| 4 | 15.96 | 16.25 | 16.39 | 16.35 | 15.89 | 15.36 | 16.03 +/− 0.3883 (2.42) |
| 5 | 19.1 | 19.66 | 19.28 | 19.73 | 19.19 | 19.16 | 19.35 +/− 0.2719 (1.40) |
| 6 | 22.52 | 23.06 | 23.03 | 23.13 | 23.04 | 22.39 | 22.86 +/− 0.3196 (1.40) |
| 7 | 25.7 | 25.79 | 26.76 | 26.76 | 26.89 | 25.72 | 26.27 +/− 0.5869 (2.23) |
| 8 | 29.69 | 29.46 | 28.92 | 28.37 | 29.57 | 29.21 | 29.20 +/− 0.4923 (1.69) |
a
Standard deviation
b
Coefficient of variation (%)