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Comparison experiments to determine the stool detection limit of ELISA for rotavirus vs. real time PCR quantitation of the VP6 gene
| Standard plasmid copy | Cq values | FFU/ml (WC3 vaccine virus strain) | Cq values | Stool detection ELISA results |
|---|---|---|---|---|
| 7.90E+07 | 11.85 | 1.00E+07 | 12.04 | Positive |
| 7.90E+06 | 15.76 | 1.00E+06 | 16.96 | Positive |
| 7.90E+05 | 19.24 | 1.00E+05 | 19.77 | Positive |
| 7.90E+04 | 24.2 | 1.00E+04 | 23.52 | Negative |
| 7.90E+03 | 28.97 | 1.00E+03 | 29.8 | Negative |
| 7.90E+02 | 32.84 | 1.00E+02 | 33.01 | Negative |
