Table 2.

Biochemical analysis of AtzD

Inhibitor/treatment	Residual activity (%)
PMSF	8.5
o-Phenanthroline	1.2
EDTA	100
ApoAtzD plus Mg2+ (50 μM)a	60
Lysine alkylation	7.5

^aApoAtzD treated by the addition of 50 μM Zn²⁺, Na⁺, K⁺, Mn²⁺, Co²⁺, Cu²⁺, Ca²⁺ or Fe²⁺ failed to recover any catalytic activity.