Table 5.
Comparison of the differential phenotypes expressed by C. sakazakii SP291 and C. sakazakii ATCC BAA-894 related to antimicrobial and toxic compounds as determined by phenotype microarray.
| Test compound | Differencea | Mode of action |
|---|---|---|
| PHENOTYPES GAINED BY C. sakazakii SP291 RELATIVE TO C. sakazakii ATCC BAA-894- | ||
| Amoxicillin | 20,446 | Wall, lactam |
| Neomycin | 47,681 | Protein synthesis, 30S ribosomal subunit, aminoglycoside |
| Sisomicin | 40,297 | Protein synthesis, 30S ribosomal subunit, aminoglycoside |
| Tobramycin | 33,297 | Protein synthesis, 30S ribosomal subunit, aminoglycoside |
| Sodium arsenate | 22,155 | Toxic anion, PO4 analog |
| Sodium metaborate | 65,231 | Toxic anion |
| EDTA | 89,134 | Chelator, hydrophilic |
| 5,7-Dichloro-8-hydroxyquinoline | 21,638 | Chelator, lipophilic |
| 5-Nitro-2-furaldehyde semicarbazone | 40,348 | DNA damage, multiple sites, nitrofuran analog |
| Protamine sulfate | 27,720 | Membrane, non-specific binding |
| Streptomycin | 40,297 | Protein synthesis, 30S ribosomal subunit, aminoglycoside |
| Potassium tellurite | 27,025 | Toxic anion |
| Sodium tungstate | 50,849 | Toxic anion, molybdate analog |
| Poly-L-lysine | 43,324 | Membrane, detergent, cationic |
| Sodium m-arsenite | 28,927 | Toxic anion |
| Sodium periodate | 47,820 | Toxic anion, oxidizing agent |
| Antimony (III) chloride | 35,457 | Toxic cation |
| Iodonitro tetrazolium violet | 22,427 | Respiration |
| Hexamminecobalt (III) Chloride | 30,202 | DNA synthesis |
| Apramycin | 43,717 | Protein synthesis, 30S ribosomal subunit, aminoglycoside |
| Ornidazole | 22,594 | Protein glycosolation |
| PHENOTYPES LOST BY C. sakazakii SP291 RELATIVE TO C. sakazakii ATCC BAA-894- | ||
| Cloxacillin | −20,939 | Wall, lactam |
| Colistin | −35,638 | Membrane, transport |
| Guanidine hydrochloride | −47,347 | Membrane, chaotropic agent |
| Cefmetazole | −21,713 | Wall, cephalosporin second generation |
| Phleomycin | −45,183 | DNA damage, oxidative, ionizing ratiation |
| Methyl viologen | −81,565 | Oxidizing agent |
| Sodium azide | −22,375 | Respiration, uncoupler |
| Dichlofluanid | −42,205 | Fungicide, phenylsulphamide |
| Cinoxacin | −18,744 | DNA unwinding, gyrase (GN), topoisomerase (GP), quinolone |
| Rifamycin SV | −22,130 | RNA polymerase |
| Glycine hydroxamate | −64,946 | tRNA synthetase |
| D,L−Methionine hydroxamate | −38,317 | tRNA synthetase |
| Sodium bromate | −27,123 | Toxic anion |
| Guanazole | −33,164 | Ribonucleotide DP reductase |
| Ciprofloxacin | −22,172 | DNA unwinding, gyrase (GN), topoisomerase (GP), fluoroquinolone |
| Tolylfluanid | −18,317 | Fungicide, phenylsulphamide |
a
Denotes the following: a positive number indicates faster growth in C. sakazakii SP291 compared to C. sakazakii ATCC BAA-894; a negative number indicates faster growth in C. sakazakii ATCC BAA-894 compared to C. sakazakii SP291.