Figure 1.

Nitric oxide (NO) and in vivo thiol pool measurement in cold. (A) NO production measured using NO measuring system after the cold treatment (2–96 h) to the seedlings. (B) NO production in the extract from control (room temperature, RT) and cold (4°C) treated seedlings with L-arginine (L-Arg, 1 mM) alone or with NADPH (1 mM), L-NAME (1 mM), nitrite (1 mM) alone or with NADH (1 mM) and tungstate (1 mM). (C) Alterations in the thiol pool in cold (6 h) treated seedlings. Low mol. wt. thiols; low molecular weight thiols. Results represent mean ± SD from three independent experiments performed in triplicates. Asterisk (*) indicates significant differences between control and cold with p = 0.05 calculated using Student's t-test.