Table 2.
Cold responsive S-nitrosylated proteins from RuBisCO depleted fractions of cold treated B. juncea seedlings.
| Protein name | Acc. no. | M.S. | S.C.(%) | Matched peptides [Identified peptide (ion score)] | MW(Da)/pl | Spot/polypeptide number and patter | Reported as S-nitrosylated in crude | Reported as S-nitrosylated protein in anyother study | Functional category | |
|---|---|---|---|---|---|---|---|---|---|---|
| Observed | Theoretical | |||||||||
| Putative lactoylglutathionelyase (Brassica rapa) | gi|157890952 | 487 | 36% | 10[IANQELGGKITR (84)] | 15657/6.2 | 32006/5.3 | 2, U | No | – | Stress/signaling/redox |
| Daikon cysteine protease RD21 (Raphanus sativus) | gi|219687002 | 246 | 16% | 5[NGGIDTEEDYPYK (105)] | 22055/5.5 | 32085/4.27 | 4, U | No | Maldonado-Alconada et al., 2010 | |
| Vacuolar calcium binding protein (Raphanus sativus) | gi|9049359 | 120 | 9% | 2[TEETPAVVEEEK (61)] | 29003/6.6 | 27094/4.1 | 8, D | No | – | |
| Fe-superoxide dismutase, partial (Arabidopsis thaliana) | gi|166700 | 50 | 6% | 2[RPDYIK (28)] | 41087/6.7 | 25409/6.3 | 11, U | Yes | Lin et al., 2012; Tanou et al., 2012 | |
| Glutathione S-transferase (Brassica napus) | gi|87294807 | 160 | 17% | 4[SPLLLQSNPIHK(52)] | 95510 | 24887 | 3, U | Yes | Tanou et al., 2009 | |
| Chaperonin 10 (Arabidopsis thaliana) | gi|3057150 | 77 | 8% | 3[YAGTEVEFNDVK (49)] | 95510 | 26912 | 3, U | No | Lindermayr et al., 2005 | |
| Epithiospecifier protein (Brassica rapa) | gi|211905345 | 428 | 33% | 16[FITKLDEEGGPEAR(74)] | 53640 | 37890 | 6, U | No | – | |
| MLP-like protein 328 (Arabidopsis thaliana) | gi|18379240 | 108 | 17% | 7[GLEGHVMEQLK(47)] | 40430 | 17616 | 8, U | Yes | Lindermayr et al., 2005 | |
| Soluble inorganic pyrophosphatase 1 (Arabidopsis thaliana) | gi|15242465 | 222 | 11% | 3[MEVATDEDFTPIK(90)] | 29010/7 | 33644/5.7 | 7, D | No | – | Metabolism |
| Putative fructose-bisphosphate aldolase (Arabidopsis thaliana) | gi|14539316 | 176 | 11% | 3[LDSIGLENTEANR(94)] | 43041/6.9 | 37291/8.8 | 12, U | Yes | Lindermayr et al., 2005; Maldonado-Alconada et al., 2010; Tanou et al., 2012 | |
| Fructose-bisphosphate aldolase, class I (Arabidopsis thaliana) | gi|18420348 | 713 | 19% | I5[LDSIGLENTEANR(116)] | 52132/7 | 43029/6.7 | 14, U | Yes | Lindermayr et al., 2005 | |
| AT4g38970/F19H22_70 (Fructose biphosphate aldolase 1, Arabidopsis thaliana) | gi|16226653 | 334 | 32% | I8[LDSIGLENTEANR(47)] | 53640 | 38858 | 6, U | Yes | Lindermayr et al., 2005 | |
| Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (Sinapinis alba) | gi|120675 | 599 | 59% | 25[VPTVDVSWDLTVR(75)] | 53640 | 37015 | 6, U | No | Lindermayr et al., 2005; Maldonado-Alconada et al., 2010 | |
| Sedoheptulose-bisphosphatase (Arabidopsislyrata) | gi|297816906 | 675 | 27% | 18[LTGVTGGDQVAAAMGIYGPR (134)] | 68081/6.2 | 42861/6.0 | 15, U | Yes | Tanou et al., 2009 | Photosynthesis |
| Sedoheptulose-bisphosphatase (Arabidopsislyrata) | gi|297816906 | 358 | 22% | 11[GIFTNVTSPTAK(70)] | 42250 | 42861 | 7, U | Yes | Tanou et al., 2009 | |
| Beta-carbonic anhydrase, chloroplastic (Brassica napus) | gi|297787439 | 374 | 29% | 11[VISELGDSAFEDQ CGR(82)] | 95510 | 36127 | 3, U | No | Lindermayr et al., 2005; Tanou et al., 2012 | |
| Oxygen-evolving enhancer protein 2, chloroplastic (Pisum sativum) | gi|131390 | 169 | 10% | 7[FVEDTASSFSVA(76)] | 37500 | 28201 | 9, U | No | Lindermayr et al., 2005 | |
| Hypothetical protein SORBIDRAFT_02g002690, (23 kDa polypeptide of PS II) | gi|242047384 (AAB82135) | 122 | 8% | 3[HQLITATVSDGK(63)] | 37500 | 27718 | 9, U | No | Tanou et al., 2012 | |
| Unknown protein 18 (Vitis rotundifolia) | gi|205830697 | 149 | 100% | 3[TNAENEFVTIKK((78)] | 29950/5.3 | 1393/5.85 | 9. D | No | – | Unknown |
| Unknown protein 18 (Vitis rotundifolia) | gi|205830697 | 158 | 100% | 1[TNAENEFVTIK(80)] | 22076/5.8 | 1393/5.85 | 5, D | No | – | |
| Unnamed protein product (Thellungiella halophila) | gi|312282755 | 500 | 53 | 2[(VPTVDVSWDLTVR(75)] | 53640 | 32088 | 6,U | No | – | |
Protein identification was performed by nLC-MS/MS using Mascot search engine using NCBI database. Spots/polypeptides were identified from Figure 5B and Supplementary material S1. Proteins in bold indicate novel S-nitrosylation targets which are not identified in any plant system. Proteins in italics indicate the protein identified from 1-D gel. The best matching peptide identifying the protein with ion score is reported, M.S., Mowse Score; Acc. no., Accession number; Seq. Cov., Sequence coverage.