Table 1.
Components | Amount |
---|---|
Destination vector (e.g. pGoldenGate-SE7) | 100 ng |
Each additional assembly piece | In equal molar concentration to destination vector |
10X NEB T4 DNA ligase buffer | 1.5 μl |
10 mM ATP | 1.5 μl |
BsaI or high concentrated SapI | 1.0 μl |
NEB T4 DNA ligase (2 million cohesive end units/ml) | 1.0 μl |
dH2O | To bring the total volume to 15 μl |