Table 1.

Assembly of Golden Gate reaction.

Components	Amount
Destination vector (e.g. pGoldenGate-SE7)	100 ng
Each additional assembly piece	In equal molar concentration to destination vector
10X NEB T4 DNA ligase buffer	1.5 μl
10 mM ATP	1.5 μl
BsaI or high concentrated SapI	1.0 μl
NEB T4 DNA ligase (2 million cohesive end units/ml)	1.0 μl
dH2O	To bring the total volume to 15 μl