Table 2.
37°C 2 min. | 25–50X |
16°C 5 min. | |
80°C 10 min. | |
‡37°C 30 min. |
Before the start of this step, 1 μl of fresh BsaI needs to be added to the reaction mix.
37°C 2 min. | 25–50X |
16°C 5 min. | |
80°C 10 min. | |
‡37°C 30 min. |
Before the start of this step, 1 μl of fresh BsaI needs to be added to the reaction mix.