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. Author manuscript; available in PMC: 2013 Sep 2.
Published in final edited form as: Nature. 2012 Dec 16;493(7432):406–410. doi: 10.1038/nature11725

Fig. 3. The effect of mutant PPM1D isoforms on p53 activation.

Fig. 3

p53 wildtype U2-0S human osteosarcoma cells were transfected with PPM1D cDNA expression constructs and exposed to ionising irradiation (5 grays). At 30 minute and 4h intervals whole cell lysates were western blotted to estimate the IR-induced activation of p53. Western blots showing p53 and actin (loading control) protein levels at different times (in hours) after IR exposure are shown. ‘Empty’: transfected with empty construct, ‘PPM1D WT’: transfected with wildtype PPM1D construct, ‘PPM1D c.1384C>T’ and ‘PPM1D c.1420delC’: transfected with mutant PPM1D constructs. Suppression of p53 was enhanced in cells transfected with mutant constructs suggesting these alleles encode hyperactive PPM1D isoforms.