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. 2013 Sep 2;3:2544. doi: 10.1038/srep02544

Figure 4. Effect of fnr1, fnr2 and fnr3 mutations on expression of the fixNOP and hemN-Hsero_3206-fixIS operons in H. seropedicae.

Figure 4

(a) Schematic representation of genomic region encoding the fixNOP and hemN-Hsero_3206-fixIS operons. The genes and predicted functions in the locus are: Hsero_3198, transmembrane protein; Hsero_3199, FixH domain containing protein; fixG, iron-sulfur 4Fe-4S ferredoxin transmembrane protein; fixP, cbb3-type cytochrome c oxidase-subunit III; fixO, cbb3-type cytochrome oxidase-subunit II; fixN,cbb3- type cytochrome c oxidase, subunit I; fixS, nitrogen fixation protein P-type ATPase protein; fixI, cation transport P-type ATPase protein; Hsero_3206, conserved hypothetical protein; hemN, oxygen-independent coproporphyrinogen III oxidase. Black rectangles represent putative FNR-boxes. Genes are not drawn to scale. (b) β-Galactosidase activities of fixN::lacZ and hemN::lacZ fusions incubated for 3 hours under the oxygen concentrations of 2.0% (black bars), 4.0% (dark grey bars), 6.0% (light grey bars) and 20.8% (white bars). CTRL indicates the SmR1 strain carrying the vector plasmid pPW452 (which contains the lacZ gene without a promoter).