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. 2013 Sep 2;3:2544. doi: 10.1038/srep02544

Figure 6. Influence of Fnr and FixN on nitrogenase activity.

Figure 6

The acetylene reduction assay was performed as described in Methods using strains grown in (a) semi-solid medium supplemented with 0.5 mM of sodium glutamate or (b) liquid medium supplemented with 0.5 mM of ammonium chloride under 5% initial oxygen in the gas phase. In (b) samples were taken from the culture to measure the growth curve (primary y axis) of SmR1 (black squares), RAM 21 (dark grey triangles) and MB231 (light grey circles). Black, dark grey and light grey bars (secondary y axis) indicate the nitrogenase activity of SmR1, RAM21 and MB231, respectively. Data represent the average of two independent experiments performed in duplicate. Error bars indicate standard deviations.