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. 2013 Sep 2;3:2567. doi: 10.1038/srep02567

Figure 3. Loss of the central ISTID region stabilises Nmnat2-Venus in vivo.

Figure 3

(a) Representative Western Blots (cropped) of Nmnat2-Venus expression in sciatic nerves from Nmnat2-Venus line 2 animals 24 or 72 hours after axotomy compared to contralateral, uncut nerve from the same animal. Nmnat2-Venus was detected by the YFP tag. βIII-Tubulin was used as loading control. (b) Representative Western Blot (cropped) of Nmnat2Δex6-Venus expression (detected by YFP tag) in sciatic nerve 72 hours after axotomy compared to contralateral, uncut nerve from the same animal. βIII-Tubulin was used as loading control. (c) Quantification of Nmnat2-Venus and Nmnat2Δex6-Venus turnover in cut sciatic nerve. Expression of Nmnat2-Venus (or Nmnat2Δex6-Venus) was normalised to βIII-Tubulin levels in the same nerve. For presentation purposes, expression levels at 24 and 72 hours after axotomy were normalised to uncut nerve. Error bars indicate SEM. *** indicates statistically significant difference compared to Nmnat2-Venus (p < 0.001, n = 3–4 nerves per line).