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Increased frequency and number of Treg cells in patients treated with sirolimus monotherapy. (A) Frequency and (B) number of FoxP3+CD4+ T cells in patients divided according to maintenance immunosuppression regimen. Cells were gated as FoxP3+CD4+CD3+7-AAD−. (C) Quantitative PCR measurement of FoxP3 mRNA in peripheral blood. Data expressed in arbitrary units calculated using the 2−ΔΔCt method normalized to HPRT expression.
