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. Author manuscript; available in PMC: 2013 Sep 2.

Published in final edited form as: Transplantation. 2011 Apr 15;91(7):744–750. doi: 10.1097/TP.0b013e31820c85dc

CCR6⁺CD4⁺ T cells may be expanded in vitro and retain IL-17A production. (A) Example of intracellular cytokine staining for IL-17A and IFNγ of expanded CCR6⁻ CD4⁺ (left) and CCR6⁺CD4⁺ T cells (right panel). (B) Percentage of cytokine producing cells measured by intracellular cytokine staining of expanded CCR6⁻CD4⁺ and CCR6⁺CD4⁺ T cells (left panels). Supernatant levels of IL-17A and IFNγ from expanded CCR6⁻CD4⁺ and CCR6⁺CD4⁺ T cells (right panels). (C) Expression of CCR6, CD25, CD62L and FoxP3 on CCR6⁺CD4⁺ T cells during in vitro expansion.

CCR6⁺CD4⁺ T cells may be expanded in vitro and retain IL-17A production. (A) Example of intracellular cytokine staining for IL-17A and IFNγ of expanded CCR6⁻ CD4⁺ (left) and CCR6⁺CD4⁺ T cells (right panel). (B) Percentage of cytokine producing cells measured by intracellular cytokine staining of expanded CCR6⁻CD4⁺ and CCR6⁺CD4⁺ T cells (left panels). Supernatant levels of IL-17A and IFNγ from expanded CCR6⁻CD4⁺ and CCR6⁺CD4⁺ T cells (right panels). (C) Expression of CCR6, CD25, CD62L and FoxP3 on CCR6⁺CD4⁺ T cells during in vitro expansion.