Fig. 4.

NSBP-1 translocated into the nucleus in the absence of insulin signaling. a–d NSBP-1::GFP accumulated in intestinal nuclei when DAF-2 activity was reduced. a, b NSBP-1::GFP in the wild type. c, d NSBP-1::GFP in daf-2 mutants. e–h Alanine substitution of a predicted AKT phosphorylation site within NSBP-1 (T203A) resulted in nuclear accumulation of NSBP-1::GFP. E, NSBP-1::GFP in the wild type (f is its DIC image), g NSBP-1T203A::GFP in the wild type (h is its DIC image). White arrows indicate the intestinal nuclei. a–h A representative image from one of three independent experiments is shown (n = 30). i–k Quantitation of NSBP-1::GFP and NSBP-1T203A::GFP in intestinal nuclei; i indicates high, j indicates medium, and k indicates low level of NSBP-1::GFP in intestinal nuclei