Fig. 6.

NSBP-1 regulates DAF-16-dependent transcription. a Decreased sod-3, mtl-1, and sbp-1 mRNA in daf-2 and wild-type worms treated with nsbp-1-RNAi. qRT-PCR was performed to assess gene expression. *p ≤ 0.05 versus control, NS not significant. b Overexpression (O/E) of nsbp-1 extended lifespan. Mean lifespan of the wild type 17.2 ± 0.1 (n = 96); nsbp-1(O/E) 21.6 ± 0.5 (n = 107), p = 0.0025; nsbp-1 T203A 22.0 ± 0.5 (n = 164), p < 0.0001; mean lifespan of daf-16 13.3 ± 0.2 (n = 108); daf-16; nsbp-1(O/E) 13.0 ± 0.5 (n = 133), p = 0.63. c Overexpression (O/E) of nsbp-1 increased resistance to oxidative stress. Worms (n = 30 for each strain) were treated with paraquat, and then survival was assessed. In the wild-type background, survival rate was nsbp-1(+) 61 %; nsbp-1(O/E) 78 %, p < 0.001 at 20 h. In the daf-16 background, nsbp-1 overexpression did not improve survival rate. p ≤ 0.001 versus nsbp-1(+), NS not significant. d–g Fat storage measured by Oil-Red O staining. Fat storage in both the wild type (d–f) and daf-16 (g–h) increased when nsbp-1 was overexpressed. A representative image from one of three independent experiments (n = 20) is shown. i nsbp-1(O/E) upregulated sod-3, and this upregulation depended on DAF-16 activity. sod-3 expression determined by qRT-PCR. *p ≤ 0.05 versus each control, NS not significant