Table 1. Kinetic Parameters from a Fit of the Model to Data Where C0 = 1.89 mg/mL.
parameter | value | source |
---|---|---|
kU (s–1) | 4.0 ± 0.4 × 10–3 | a |
k–C (s–1) | 3.83 ± 0.04 × 10–3 | b |
kC (s–1) | 6 ± 4 × 10–4 | c |
k1,1 (mg–1 mL s–1) | 1.73 ± 0.04 × 10–2 | b |
kA,L (mg–2/3 mL2/3 s–1) | 0.82 ± 0.05 × 10–2 | b |
CS (mg mL–1) | 2.7 ± 0.2 × 10–5 | b |
C* (mg mL–1) | 0.113 ± 0.001 | c |
K1,1 (mg mL–1) | 0.115 | d |
k–1,1 (s–1) | 0.150 | d |
Obtained from fits of fluorescence unfolding.
Obtained from a fit to the stopped-flow data where C0 = 1.89 mg mL–1 (Figure 6B).
Obtained from a fit to the solubility data where C0 = 2.0 mg mL–1 (Figure 4).
Derived from the other kinetic parameters using eq 1: K1,1 = [CS(k–C2 + 2k–CkC + kC)]/[(CS – C*)2kC2].