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. 2013 Mar 19;135(17):6456–6464. doi: 10.1021/ja308852b

Table 1. Kinetic Parameters from a Fit of the Model to Data Where C0 = 1.89 mg/mL.

parameter value source
kU (s–1) 4.0 ± 0.4 × 10–3 a
k–C (s–1) 3.83 ± 0.04 × 10–3 b
kC (s–1) 6 ± 4 × 10–4 c
k1,1 (mg–1 mL s–1) 1.73 ± 0.04 × 10–2 b
kA,L (mg–2/3 mL2/3 s–1) 0.82 ± 0.05 × 10–2 b
CS (mg mL–1) 2.7 ± 0.2 × 10–5 b
C* (mg mL–1) 0.113 ± 0.001 c
K1,1 (mg mL–1) 0.115 d
k–1,1 (s–1) 0.150 d
a

Obtained from fits of fluorescence unfolding.

b

Obtained from a fit to the stopped-flow data where C0 = 1.89 mg mL–1 (Figure 6B).

c

Obtained from a fit to the solubility data where C0 = 2.0 mg mL–1 (Figure 4).

d

Derived from the other kinetic parameters using eq 1: K1,1 = [CS(k–C2 + 2k–CkC + kC)]/[(CSC*)2kC2].