Figure 1.
Electro gene transfer performed with the T820 electroporator (BTX, San Diego, CA, USA). (a) 25 μg of plasmids diluted in 20 μL of 0.9% NaCl with 6 mg/mL polyglutamate were injected in each of the tibial muscles of anesthetized mice; (b) Two square-wave 25 ms, 375 V/cm pulses generated by the T820 electroporator were applied by two electrodes placed on the shaved skin.