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. Author manuscript; available in PMC: 2013 Sep 2.
Published in final edited form as: Biotechniques. 2012 Aug;0(0):1–5. doi: 10.2144/000113909

Figure 1. Human Cytomegalovirus Major Immediate Early Enhancer/Promoter constructs used for attenuated gene expression.

Figure 1

The human CMV promoter structure is shown above, with the transcription start site at the +1 position and (putative) upstream binding sites for different transcription factors. Promoter deletion positions are shown below. Transcription factor binding elements were identified using the TESS analysis tool (http://www.cbil.upenn.edu/cgi-bin/tess/tess?RQ=WELCOME, (22)), with a consensus sequence cutoff of >12.0. Similar but slightly different variants of this binding site map were also generated by the program TFSEARCH (http://www.cbrc.jp/research/db/TFSEARCH.html, (23)) and have been published (24). Promoter deletions were introduced by cloning PCR fragments MluI-XbaI sites) with the designated deletions into a pcDNA™3.1/myc-His(-)A expression vector (Life Technologies) that carried a custom multicloning site between the XbaI and AflII sites. Deletion design and DNA sequences are provided in Supplemental Fig. 1, and construct numbers are provided in Supplemental Table 1.