Figure 4.

Deletion of Rictor does not alter T_reg function but partially rescues inflammation in Foxp3^creRaptor^fl/fl mice. a, T_reg percentage in 2–3 month old wild-type and Foxp3^creRictor^fl/fl mice. b, Expression of CD62L and CD44 on splenic T cells from wild-type and Foxp3^creRictor^fl/fl mice. c, T_regs were activated by anti-CD3 and IL-2 for 4 hours followed by immunoblots. d, Expression of CD62L and CD44 expression on splenic CD4⁺ T cells from 3–4 week old wild-type, Foxp3^creRaptor^fl/fl and Foxp3^creRaptor^fl/flRictor^fl/fl mice. Right, percentage of CD62L^hiCD44^lo naïve CD4⁺ cells in the spleen. e, IFN-γ production in splenic CD4⁺ T cells from wild-type, Foxp3^creRaptor^fl/fl and Foxp3^creRaptor^fl/flRictor^fl/fl mice. f, Numbers of total TCRβ⁺ cells in peripheral lymph nodes from wild-type, Foxp3^creRaptor^fl/fl and Foxp3^creRaptor^fl/flRictor^fl/fl mice. g, In vitro suppression assays mediated by T_regs from 3–4 week old wild-type, CD4^creRaptor^fl/fl and CD4^creRaptor^fl/flRictor^fl/fl mice. Error bars represent s.d. (n=3). h, CTLA-4 expression in T_regs from the spleen of wild-type, CD4^creRaptor^fl/fl and CD4^creRaptor^fl/flRictor^fl/fl mice. P values are determined by Mann Whitney test (a) and ANOVA (d, f, g). Results represent 3 (a-c, e-g) and 2 (d, h) independent experiments.