Skip to main content
. Author manuscript; available in PMC: 2014 Jul 31.
Published in final edited form as: Toxicol Lett. 2013 Jun 2;221(1):1–8. doi: 10.1016/j.toxlet.2013.05.007

Figure 5. Ligand binding assay.

Figure 5

Guinea pig hepatic cytosol was incubated with indicated concentrations of PEL-2 (pelargonidin-3-O-rutinoside chloride), CYA-3 (cyanidin-3,5-di-O-glucoside chloride) or 200 nM TCDF for 1 h at room temperature in the presence of 2 nM [3H]TCDD. Ligand binding to the cytosolic proteins was determined by the hydroxyapatite binding protocol and scintillation counting. Specific binding was determined as a difference between total and non-specific (TCDF) reactions. The values are presented as mean ± SD of three independent reactions. * - values significantly different from the ‘no competitor’ reaction at P<0.05 as determined by the Student’s t-test. The results are representative of two independent experiments.