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Expression of Nhlh2 in the hypothalamus of developing and adult Nkx2.1iCre/+;Ngn3flox/flox mice. In situ hybridization reveals that Nhlh2 expression is absent in the early embryonic hypothalamus of mutant mice (A–D). In late development, a few Nhlh2-positive cells are observed per section (E,F; arrows); however, no expression is observed in the adult mutant ARC (G,H). 3v, third ventricle; Arc, arcuate nucleus.
