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. 2013 Jun 20;6(5):1167–1174. doi: 10.1242/dmm.011700

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — DNA screening and protein analyses in the Dmd^mdx/Large^myd model. (A) DNA screening for the injected cells: PCR for the Large normal allele (162 bp) in the injected mice. Animals 1 and 2 were injected with cells, and animal 3 with PBS. Columns: 1, heart; 2, gastrocnemius; 3, stomach; 4, quadriceps; 5, diaphragm; 6, tail. Positive control (162 bp) and molecular weight (MW) are shown. (B) Dystrophin western blot analysis from different muscles from the mASC-injected and PBS-injected animals, reacted with antibody to dystrophin (H300, Santa Cruz Biotechnology, Inc.). Column 1, molecular weight; 2, normal human muscle; 3, normal C57BL muscle. Muscles from Dmd^mdx/Large^myd mouse 1: column 4, gastrocnemius; 5, quadriceps; 6, diaphragm; 7, heart; 8, stomach. Muscles from Dmd^mdx/Large^myd mouse 2: 9, gastrocnemius; 10, quadriceps; 11, diaphragm; 12, heart; 13, stomach. Muscles from Dmd^mdx/Large^myd mouse 3: 14, quadriceps; 15, diaphragm. (C) Dystrophin immunofluorescence analysis in a normal control and in one injected muscle: Dmd^mdx/Large^myd mouse 2, muscle gastrocnemius.