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Effect of gene knockdown on hepatocyte gene expression and liver size. Knockdown of 8/14 genes (pnpla3, trib1, slc2a2, mapk10, cpn1, fads2, pklr and samm50) by ATG-site morpholino injection decreased the proportion of embryos with a small liver size in a statistically significant manner (A,C,E,G,I,K,M,O), as evidenced by in situ hybridization for fabp10a expression at 72 hpf (B,D,F,H,J,L,N,P).
